Development and Validation of High Performance Thin Layer
نویسندگان
چکیده
A simple, specific and precise high performance thin layer chromatographic method was developed and validated for estimation of Brimonidine tartrate as bulk drug and in ophthalmic solutions. The chromatographic development was carried out on precoated silica gel 60 F254 aluminium plates using mixture of Methanol: Ammonia (8:0.2 v/v) as mobile phase and densitometric evaluation of band was carried out at 250 nm using Camag TLC Scanner-3 with win CAT 1.4.3 version software. The Rf value of drug was found to be 0.52 ± 0.01. The method was validated with respect to linearity, accuracy, precision and robustness. The calibration curve was found to be linear over a range of 2001200 ng/ band. The % assay (Mean ± S.D.) was found to be 101.3 ± 1.02. Thus the proposed HPTLC method was found to provide a faster and cost effective quantitative control for routine analysis of brimonidine tartrate as bulk drug and in ophthalmic solutions.
منابع مشابه
High-Performance Thin-Layer Chromatographic Fingerprints of Flavonoids and Phenol Carboxylic Acids for Standardization of Iranian Species of the Genus Crataegus L.
Eight samples of flowering tops from six species of the genus Crataegus L., commonly called Hawthorn, from different geographic locations of Iran were standardized according to German Pharmacopoeia monograph on Crataegi folium cum flore (hawthorn leaf with flower) by high-performance thin-layer chromatograph-ic (HPTLC) fingerprinting combining with digital scanni...
متن کاملPerformance Model for Vertical Wells with Multi-stage Horizontal Hydraulic Fractures in Water Flooded Multilayer Reservoirs
For the characteristics of horizontal fractures in shallow low-permeability oil layers after hydraulic fracturing in multilayer reservoirs, horizontal fractures are taken equivalent to an elliptical cylinder with the reservoir thickness using the equivalent permeability model; then, upon the elliptic seepage theory, the seepage field which has led by a vertical well with horizontal fractures is...
متن کاملInfluence of Sodium Tripolyphosphate Concentration on Characteristics and Performance of Polyamide Thin Layer Membrane in Cu (II) Removal
In this work, the effect of presence of the sodium tripolyphosphate (STPP), as an inorganic salt, on improving the performance of polyamide (PA) thin layer membranes has been studied. Characterization analyses confirmed the presence of the salt on the whole surface structure of the thin layer. Different salt loadings resulted in different fluxes and Cu (II) rejections. The thin layer containing...
متن کاملPerformance and Structure of Thin Film Composite Reverse Osmosis Membranes Prepared by Interfacial Polymerization in the Presence of Acid Acceptor
During interfacial polymerization (IP) reaction between m-phenylenediamine (MPDA) and trimesoyl chloride (TMC), a by-product, i.e. hydrochloric acid can produce. This produced acid diffuses back in aqueous phase and protonates MPDA and reduces its reactivity that results in lowering of polymer yield and performance of membrane. Further, for getting consistency in reverse...
متن کاملPolyamide Forward Osmosis Membrane: Synthesis, Characterization and Its Performance for Humic Acid Removal
In this research, modification on the ultrafiltration (UF) membrane by synthesis of a thin layer of polyamide selective layer was designed for high performances of forward osmosis (FO) water treatment. Two monomers, m-Phenylenediamine (MPD) and Trimesoyl chloride (TMC) with different concentrations of MPD (2.0% w/v and 1.0% w/v) were reacted with TMC (0.15% w/v) for interfacial polymerization (...
متن کاملA Validated HPTLC-Densitometric Method for Assay of Aucubin in Vitex agnus-castus L.
Vitex agnus-castus L. is a medicinal plant which is used in several dosage forms for women hormonal disorders and standardized according to the iridoids or flavonoids content. Aucubin, an iridoid glycoside, considered as a marker in some formulations. In this research, a thin layer chromatographic method with densitometric detection has been developed for quantitative determination of aucubin i...
متن کامل